Nina, thinking it was a lolly, swallows an object called "Crystal Pearl". But the candy turns out to be a test for the magicians. To retrieve the crystal, a special medicine has to be made so now Zero and Ichii, the magicians, have to protect her from others while waiting for the medicine to be completed.

With its gorgeous landscape and prosperous people, Great Japan is the envy of all other nations. But a serious threat hovers over the country. Mysterious guardians known as Master Samurai are Great Japan's only defense. At the behest of the student council, young samurai Muneakira Yagyuu arrives at Buou Academic School. Run by the Tokugawa Shogunate, here children of warriors are given aristocratic education required to run the country. The school is led by the student council president Yoshihiko Tokugawa and his sister Sen, who also happens to be Muneakira's childhood friend. Upon arriving at the academy, Muneakira finds himself in the midst of a terrible fight. During the chaos, the sky fills with a peculiar white light and a mysterious girl named Juubei Yagyuu appears and suddenly kisses Muneakira. With his kiss, she awakens an unknown power that protects them. Just who is this girl, and where did she come from? Muneakira finds himself entangled in the fate of the country and a threat that will shake Great Japan to its core. He must learn the secret behind the Master Samurai and the kiss that awakened Juubei's power in order to protect his country.

Second season of Hyakka Ryoran: Samurai Girls. The series takes place in an alternate version of Japan called Great Japan (大日本国, Dai Nippon-koku), in an alternate timeline where the Tokugawa shogunate remained active and has remained semi isolated from the rest of the world after their defeat by American forces in World War II. And the Americans have bases on Okinawa and southern great Japan. The story takes place at Buou Academic School (武應学園塾, Buō Gakuen-juku), an academy located at the base of Mount Fuji where children of military families train to become samurai warriors. The academy is in the middle of a power struggle between Yukimura Sanada and Matabei Goto of the Toyotomi faction and the powerful student council that rules the school, and Muneakira Yagyu, the owner of the Yagyu Dojo, and the mysterious Jubei Yagyu are dragged in the middle of the conflict.

Seventeen-year-old Marco Polo leaves his home in Venice on a trip to the little-known country of "Yuan", in the company of his father Nicolo and his uncle Matteo. Their mission is to deliver the sacred oil from the Tomb of Christ and a letter from the Pope to the Kublai.